Oval squid Sepioteuthis lessoniana hatchlings originated from maternally identical egg strings were incubated under constant temperature and examined with special reference to statolith formation. Statoliths of a hatchling already had an adult-like shape, and some increments were observed. In spite of the maternally identical origin and constant incubation temperature (25°C), dorsal mantle length and statolith length of the hatchlings varied from 4.70 to 6.26 mm and 320 to 410 μm, respectively (N=14). These variations had a positive correlation with the embryonic duration ranging from 22 to 27 d, which means that the size of body and statolith becomes larger in hatchlings hatching later.